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Test Code WBCWD WBC with Differential LAB334

Test Method

Sysmex XN Systems:

  • WBC:  Impedance counting
  • WBC Differential: the instrument makes 3 measurements (volume,
    conductivity and scatter) as each cell passes through the flow
    cell. The low frequency impedance measurement defines cell volume.
    The high frequency conductivity measurement indicates the internal
    conductivity. The light scatter measurement indicates the structure
    and shape. An algorithm is applied to determine different cell
    populations.
  • Abs Neutrophils, Abs Lymphocytes, Abs Monocytes, Abs
    Eosinophils, Abs Basophils:
    Calculated Parameters [Total WBC x (%Diff
    Analyte Result/100)] = Absolute Diff Analyte Count

Specimen Requirement

EDTA whole blood is required.

Reference Values

WBC:

0-1 Year, Male and
Female: 6.0-17.5 thou/mcL

  • Critical Value:

    •  
    • High: > 50.0
    •  
    • Low: < 1.0

1-2 Years, Male and Female: 6.0-17.0
thou/mcL

  • Critical Value:

    •  
    • High: > 50.0
    •  
    • Low: < 1.0

2-4 Years, Male and Female:
5.5-15.5 thou/mcL

  • Critical Value:

    •  
    • High: > 50.0
    •  
    • Low: < 1.0

4-6 Years, Male and Female:
5.0-14.5 thou/mcL

  • Critical Value:

    •  
    • High: > 50.0
    •  
    • Low: < 1.0

6-8 Years, Male and Female:
4.5-13.5 thou/mcL

  • Critical Value:

    •  
    • High: > 50.0
    •  
    • Low: < 1.0

8-10 Years, Male and Female: 4.5-13.5
thou/mcL

  • Critical Value:

    •  
    • High: > 50.0
    •  
    • Low: < 1.0

10-16 Years, Male and Female:
4.5-13.0 thou/mcL

  • Critical Value:

    •  
    • High: > 50.0
    •  
    • Low: < 1.0

Adult Male and Female: 3.7-11.0
thou/mcL

  • Critical Value:

    •  
    • High: > 50.0
    •  
    • Low: < 1.0

Neutrophils: 42.0-74.0%

Lymphocytes: 18.0-44.0%

Monocytes: 5.0-13.0%

Eosinophils: 00-8.0%

Basophils: 0.0-1.0%

Absolute Neutrophils: 1.6-6.1 thou/mcL

  • Critical Value: Low: < 0.5 thou/mcL

Absolute Monocytes: 0.2-0.8 thou/mcL

Absolute Eosinophils: 0.0-0.5 thou/mcL

Absolute Basophils: 0.0-0.1 thou/mcL

Absolute IG: 0.0-0.1 thou/mcL

 

 

Performance

Testing is performed Monday through Sunday.

Routine orders: Typically completed within 4 hours after the
specimen arrives at the testing site.

Expedite orders: Typically completed within 1 hour after the
specimen arrives at the testing site.

STAT orders: Typically completed within 30 minutes after
the specimen arrives at the testing site.

CPT Code Information

85004 Blood Counts: automated differential
WBC count

LOINC Code Information

6690-2 Leukocytes [#/volume] In Blood By Automated
Count

57023-4 Auto Differential Panel-Blood

Useful For

Screening tool to confirm a hematologic disorder.

Detect an unsuspected hematologic disorder.

Monitor effects of radiation or chemotherapy.

These counts are used as clinical guides in the diagnosis and/or
monitoring of many diseases.

Additional Specimen Collection Information

Whole blood collected in EDTA purple-top tube. The
tube must be at least one quarter full to be acceptable for
testing.

Capillary specimen collected in EDTA microtube. The 
tube must contain at least 375 mcl (bottom line) to be
acceptable for testing.
Refrigerate the specimen if unable to assay within 12 hours of
collection for microtainers, and 24 hours for
Vacutainers.