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Test Code WBCWD WBC with Differential LAB334

Performing Laboratory

UnityPoint Finley Hospital

Test Method

Sysmex XN:

  • WBC:  Impedance counting
  • WBC Differential: the instrument makes 3 measurements (volume, conductivity and scatter) as each cell passes through the flow cell. The low frequency impedance measurement defines cell volume. The high frequency conductivity measurement indicates the internal conductivity. The light scatter measurement indicates the structure
    and shape. An algorithm is applied to determine different cell populations.
  • Abs Neutrophils, Abs Lymphocytes, Abs Monocytes, Abs Eosinophils, Abs Basophils:
    Calculated Parameters [Total WBC x (%Diff Analyte Result/100)] = Absolute Diff Analyte Count

Performance

Testing is performed Monday through Sunday.

Routine orders: Typically completed within 2 hours upon receipt in the lab.

STAT orders: Typically completed within 30 minutes upon receipt in the lab.

Specimen Requirement

Whole blood collected in EDTA purple-top tube. The tube must be at least one quarter full to be acceptable for testing.

Capillary specimen collected in EDTA microtube. The tube must contain at least 375 ul (bottom line) to be acceptable for testing. 

Useful For

Screening tool to confirm a hematologic disorder.

Detect an unsuspected hematologic disorder.

Monitor effects of radiation or chemotherapy.

These counts are used as clinical guides in the diagnosis and/or monitoring of many diseases.

Reference Values

WBC

sex age range expected range critical low critical high
M & F 0-1 yr 6.0-17.5 thou/mcL 1.0 thou/mcL 50.0 thou/mcL
M & F 1-2 yr 6.0-17.0 thou/mcL 1.0 thou/mcL 50.0 thou/mcL
M & F 2-4 yr 5.5-15.5 thou/mcL 1.0 thou/mcL 50.0 thou/mcL
M & F 4-6 yr 5.0-14.5 thou/mcL 1.0 thou/mcL 50.0 thou/mcL
M & F 6-8 yr 4.5-13.5 thou/mcL 1.0 thou/mcL 50.0 thou/mcL
M & F 8-10 yr 4.5-13.5 thou/mcL 1.0 thou/mcL 50.0 thou/mcL
M & F 10-16 yr 4.5-13.0 thou/mcL 1.0 thou/mcL 50.0 thou/mcL
M & F ≥ 16 yr 3.7-11.0 thou/mcL 1.0 thou/mcL 50.0 thou/mcL

Neutrophils: 42.0-74.0%

Lymphocytes: 18.0-44.0%

Monocytes: 5.0-13.0%

Eosinophils: 00-8.0%

Basophils: 0.0-1.0%

Absolute Neutrophils: 1.6-6.1 thou/mcL

  • Critical Value: Low: < 0.5 thou/mcL

Absolute Monocytes: 0.2-0.8 thou/mcL

Absolute Eosinophils: 0.0-0.5 thou/mcL

Absolute Basophils: 0.0-0.1 thou/mcL

Absolute IG: 0.0-0.1 thou/mcL

 

 

CPT Code Information

85004 Blood Counts: automated differential WBC count

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