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Test Code NBDATG Direct Coombs (DAT) Baby [LAB2045]

Important Note

This test is ordered on neonates <4 months of age. For babies >4 months old, order LAB274 Direct Antiglobulin Test. 

Test Method

AABB Standard: Agglutination of red cells with an antiserum
represents the presence of the corresponding antigen in the red
cells; Ortho Gel Cards. (AABB Technical manual. 14th edition.
Edited by V Vengelen-Tyler. Bethesda, MD, American Association of
Blood Banks 2002)


Testing is performed Monday through Sunday.

Routine: Typically completed within 4 hours after the
specimen arrives at the testing site.

STAT: Typically completed within 1 hour after the specimen
arrives at the testing site.

Useful For

Detecting possible ABO or Rh(D) incompatibility with mother’s

Demonstrates in vivo coating of RBCs with IgG.

Specimen Requirement

The only acceptable specimens are: Red blood cells collected in
EDTA or Cord Blood collected in EDTA, clot tubes, or tubes with no
additive.  A capillary or micro sample may be collected in an
EDTA microtainer. Serum/Plasma gel tubes are NOT acceptable.

CPT Information

86880 Direct Antiglobulin Test

Reference Values

Direct Antiglobulin Test:

  • IgG: Negative
  • If positive results are called to the provider