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Test Code GDATPG Direct Antiglobulin Test LAB274

Important Note

Inform the laboratory blood bank of the patient's transfusion and drug history of the past year, especially in regard to blood pressure medication, diuretics and antibiotics.

For babies <4 months of age, order Direct Comb's (DAT) Baby [LAB2045]

Test Method

AABB Standard: Agglutination of red cells with an antiserum
represents the presence of the corresponding antigen in the red
cells; Ortho Gel Cards. (AABB Technical manual. 14th edition.
Edited by V Vengelen-Tyler. Bethesda, MD, American Association of
Blood Banks 2002)


Testing is performed Monday through Sunday.

Routine: Typically completed within 4 hours after the
specimen arrives at the testing site.

STAT: Typically completed within 1 hour after the specimen
arrives at the testing site.

Useful For

Detecting possible ABO or Rh(D) incompatibility with mother’s

Demonstrates in vivo coating of RBCs with IgG or the complement
component C3d.

Specimen Requirement

The only acceptable specimens are: Red blood cells collected in
EDTA or Cord Blood collected in EDTA, clot tubes, or tubes with no
additive.  A capillary or micro sample may be collected in an
EDTA microtainer. Serum/Plasma gel tubes are NOT acceptable.

CPT Code Information

86880 Direct Antiglobulin Test

Reference Values

Direct Antiglobulin Test:

  • IgG/C3D: Negative
  • If positive cells are retested using IgG antiserum.

LOINC Code Information

1007-4 Direct Antiglobulin Test.Poly Specific Reagent [Presence]
On Red Blood Cells