Test Code HBSQAB Hepatitis B Surface Antibody [LAB3480]
Test Method
Chemiluminescent immunoassay (sandwich principle); Roche cobas Pro
Performance
Testing is performed 7 days a week Monday through Sunday.
Routine requests are completed the same day once received at the laboratory.
Stat requests are completed within 4 hours of receipt at the laboratory.
Useful For
Anti‑HBs is an antibody that is directed against the hepatitis B surface antigen. Anti‑HBs can be formed following a hepatitis B infection or after hepatitis B vaccination. Antibodies are formed against the HBsAg determinant a, which is common to all subtypes, and against subtype‑specific determinants.
Anti‑HBs tests are used within the scope of hepatitis B vaccination to check the necessity and success of vaccination. In addition, anti‑HBs tests are used to monitor the course of disease following acute hepatitis B infection. The Elecsys Anti‑HBs assay uses a mixture of purified antigens of the HBsAg subtypes ad and ay from human serum.
Reference Values
Elecsys Anti-HBs result | Result | Interpretation of results |
< 8.5 | Negative | Individual is considered to be not immune to infection with HBV. |
≥ 8.5 - <11.5 | Indeterminate | Unable to determine if anti-HBs is present at levels consistent with immunity. |
≥ 11.5 | Positive | Anti-HBs concentration detected at >10 mIU/mL. Individual is considered to be immune to infection with HBV. |
Performing Laboratory
UnityPoint The Finley Hospital
Specimen Requirements
- Serum is the specimen of choice. Gel tubes are acceptable. (Red top or Gold top).
- EDTA plasma is also acceptable. (Purple top)
- Serum samples for Hepatitis B Antibody specimens are stable for up to 8 hours at 20-25°C, 6 days at 2-8°C, and 3 months at -20°C.
- EDTA plasma samples for Hepatitis B Antibody are stable for up to 8 hours at 20-25°C, 2 days at 2-8°C, and 3 months at -20°C.