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Test Code BILTDI Bilirubin, Fractionated (Total, Direct, Indirect)

Test Method

Direct: Timed Endpoint, Diazo;Beckman DXC600

Total: Timed Endpoint, Diazo; Beckman DXC600

Indirect: Calculated

Specimen Requirement

0.5 mL plasma or serum is required.

Additional Specimen Collection Information

Collect blood in a lithium heparin, green-top or red-top tube. PST and SST (gel tubes) are acceptable. Serum or plasma should be separated from contact with the cells within 2 hours of collection. Specimens not centrifuged within 4 hours of collection may be rejected. Refrigerate the specimen if unable to assay within 8 hours of collection. Hemolyzed specimens are not acceptable. Samples with >3+ lipemia must be cleared prior to analysis. Protect from light.


Testing is performed Monday through Sunday.

Routine orders: Typically completed within 4 hours after the specimen arrives at the testing site.

STAT orders: Typically completed within 30 minutes after the specimen arrives at the testing site.

CPT Code Information

82247 Chemistry: Bilirubin; total

82248 Chemistry: Bilirubin; direct

Reference Value

≥ 5 Days of Age:

  • Total: 0.3-1.2 mg.dL
  • Direct: 0.0-0.2 mg/dL
  • Indirect: 0.0-0.8 mg/dL



Values in premature infants may be higher than in full term infants amd may reach peak concentrations at later times.

Full Term:

  • Total Birth-1 Day: < 5.8 mg.dL
  • Total 1-3 Days: < 8.2 mg/dL
  • Total 3-5 Days: < 11.7 mg/dL
  • Critical Value: Total Bilirubin (Infant-1 Month): ≥ 16.0 mg/dL