Test Code VEDOZM Vedolizumab Quantitation with Antibodies, Serum
Additional Codes
MAYO: VEDOZ
EPIC: LAB3065 Vedolizumab and anti-Vedolizumab Ab
Advisory Information
This test includes both quantitation and antibody testing on all specimens. Therapeutic thresholds for vedolizumab optimal concentrations associated with good outcomes are not well established. The American Gastroenterology Association (AGA) does not have a formal guideline on optimal thresholds for vedolizumab at this time.
If there is a known justification for performing both quantitation and antibody levels, this is the correct test to order. If there is not a known reason to perform the antibodies component, order VEDOL / Vedolizumab Quantitation with Reflex to Antibodies, Serum. VEDOL begins with quantitation and when quantitation results are 15.0 mcg/mL or less, testing for antibodies to vedolizumab will be performed.
Specimen Required
1. Patient Preparation: For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.
2. Nivolumab (Opdivo) must be discontinued at least 4 weeks prior to testing for vedolizumab quantitation in serum.
Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Specimen Volume: 1.5 mL
Collection Instructions:
1. Draw blood immediately before next scheduled dose (trough specimen).
2. Centrifuge within 2 hours of draw.
Secondary ID
603025Useful For
Assessing the unexpected loss of response to therapy with vedolizumab over time
An aid to achieving desired serum levels of vedolizumab
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
VEDOL | Vedolizumab QN, S | Yes | Yes |
VEMAB | Vedolizumab Ab, S | No | Yes |
Testing Algorithm
When this test is ordered, vedolizumab quantitation and testing for antibodies to vedolizumab will always be performed.
This test includes both quantitation and antibody testing on all specimens. The therapeutic thresholds for vedolizumab and optimal concentrations associated with good outcomes are not well established. The American Gastroenterology Association (AGA) does not have a formal guideline on optimal thresholds for vedolizumab at this time.
If there is a known justification for performing both quantitation and antibody levels, this is the correct test to order. If there is not a known reason to perform the antibodies component, order VEDOL / Vedolizumab Quantitation with Reflex to Antibodies, Serum. VEDOL begins with quantitation and when quantitation results are 15.0 mcg/mL or less, testing for antibodies to vedolizumab will be performed.
Method Name
VEDOL: Liquid Chromatography-Mass Spectrometry (LC-MS/MS)
VEMAB: Electrochemiluminescent Bridging Immunoassay
Reporting Name
Vedolizumab QN with Antibodies, SSpecimen Type
SerumSpecimen Minimum Volume
0.75 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 28 days | |
Frozen | 28 days |
Reject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
VEDOLIZUMAB QUANTITATION:
Vedolizumab lower limit of quantitation=2.0 mcg/mL
VEDOLIZUMAB ANTIBODIES:
Antibodies to vedolizumab: <9.8 ng/mL
Day(s) and Time(s) Performed
Vedolizumab Quantitation: Monday, Thursday
Vedolizumab Antibodies: Tuesday, Friday
Performing Laboratory

Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
80280
82397
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
VEDOZ | Vedolizumab QN with Antibodies, S | 90794-9 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
602807 | Vedolizumab QN, S | 90805-3 |
603298 | Vedolizumab Ab, S | 86899-2 |
603299 | VEMAB Interpretation | 59462-2 |
Forms
If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.