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Test Code SSPNL Infectious Stool Panel

Performing Laboratory

St. Luke's Hospital Cedar Rapids

Specimen Requirements

Stool specimen preserved in Cary Blair/C&S transport media.

 

Collection instructions:

1.  Collect fresh stool specimen and submit 1 gram or 5 mL into the transport medium.

2.  Place stool in preservative within 2 hours of collection

3.  Label transport medium and order in computer.

4.  Transport to laboratory, ambient or refrigerated, within 4 days.

 

Note: The detection of microbial DNA or RNA is dependent upon proper sample collection, handling, transportation, storage, and preparation.  There is a risk of false-negative results due to the presence of strains with sequence variability or genetic rearrangements in the target regions of the assays.

 

The test is not recommended as a test of cure.

 

Repeat testing should not be performed on samples collected less than 7 days apart.

 

The presence of blood or mucous in the sample may interfere with testing.

Reference Ranges

Not Detected (for all targets)

 

Day (s) Test Set Up

Monday thru Sunday; continuously.

Methodology

Multiplex Polymerase Chain Reaction (PCR)

 

A negative result should not rule-out infection inpatients with a high pretest probability for gastrointestinal infection.  The assay does not test for all potential infectious agents of diarrheal disease.

 

Positive results do not distinguish between a viable/replicating organism and the presence of a nonviable organism or nucleic acid, nor do they exclude the potential for coinfection by organisms not contained within the panel.

 

NOTE: Positive C. difficile PCR tests will reflex to Toxin EIA.

 

Test Classification and CPT Coding

87505-Gastrointestinal pathogen, includes multiplex amplified probe technique, multiple types or subtypes, 3-5 targets

87507 - Gastrointestinal pathogen, includes multiplex amplified probe technique, multiple types or subtypes, 12-25 targets.

87324 - C. difficile Toxin EIA (if PCR is positive)

Report Available

1 hr, 10 min