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Test Code FIBR Fibrinogen LAB314

Test Method

Clot Based Assay; ACL Systems

Performance

Testing is performed Monday through Sunday.

Routine orders: Typically completed within 4 hours

Expedite orders: Typically completed within 1 hour after the
specimen arrives at the testing site.

STAT orders: Typically completed within 30 minutes after
the specimen arrives at the testing site.

Useful For

Detecting increased or decreased fibrinogen (factor I)
concentration of acquired or congenital origin

Monitoring severity and treatment of disseminated intravascular
coagulation and fibrinolysis

Specimen Requirement

Sodium citrate blue-top tube filled to the area within the
indicator arrow printed on the side of the tube.

CPT Code Information

85384 Coagulopathy Testing: Fibrinogen Activity

Reference Values

200-393 mg/dL

  • Critical value: < 80 mg/dL

LOINC Code Information

3255-7 Fibrinogen [Mass/Volume] In Platelet Poor Plasma By
Coagulation Assay

Additional Specimen Collection Information

Invert the sodium citrate tube end to end 4 times to
thoroughly mix blood and anticoagulant. The tube should be left
stoppered until time of testing. Specimens stored at room
temperature should be centrifuged and tested within four hours of
specimen collection. If the testing is not completed within 4 hours
the plasma should be removed from the cells and transferred to a
plastic tube. Freeze the sample for up to two weeks. Frozen samples
should be rapidly thawed at 37°c while gently mixed. Thawed
samples should be centrifuged and tested immediately. If testing
cannot be performed immediately the sample may be held for a
maximum of 2 hours refrigerated, until tested.

  • Precautions:

    • It is not recommended to use a winged blood
      collection set (Butterfly).
    • If a vacuum tube butterfly must be used, the first tube in the
      series will be under-filled. Collect a “discard citrate
      tube”, and then collect the citrate tube used for testing.
      This will ensure the proper blood to anticoagulant ratio.
    • If using a syringe, use a BD Blood Transfer Device to transfer
      blood from the syringe to the citrate tube within one minute of
      collection. Hold the syringe with the plunger in the up-right
      position and allow the vacuum present to fill the tube. Before
      collection, be sure the syringe plunger is pushed to the top of the
      syringe. The transfer device may pop up and needs to be
      held down to completely fill tube to between fill
      lines.
    • Do not “force fill” blood into a vacuum tube. This
      may result in activation of the platelets possibly leading to
      erroneous results. Allow the vacuum present to fill the tube.
    • Do not remove vacuum tube cap to add blood.
    • Specimens should be obtained from a single venipuncture with
      minimal tissue trauma. Never combine multiple blood collections
      into a single vacuum tube.
    • Never re-use a syringe that was used for a pic line saline
      flush.

Refer to the “Citrated Plasma for Coagulation Studies” protocol
in the Quality Assurance Manual.