Test Code FIBR Fibrinogen LAB314
Test Method
Clot Based Assay; ACL Systems
Specimen Requirement
Sodium citrate blue-top tube filled to the area within the
indicator arrow printed on the side of the tube.
Additional Specimen Collection Information
Invert the sodium citrate tube end to end 4 times to
thoroughly mix blood and anticoagulant. The tube should be left
stoppered until time of testing. Specimens stored at room
temperature should be centrifuged and tested within four hours
of
specimen collection. If the testing is not completed within 4
hours
the plasma should be removed from the cells and transferred to
a
plastic tube. Freeze the sample for up to two weeks. Frozen
samples
should be rapidly thawed at 37°c while gently mixed. Thawed
samples should be centrifuged and tested immediately. If
testing
cannot be performed immediately the sample may be held for a
maximum of 2 hours refrigerated, until tested.
-
Precautions:
- It is not recommended to use a winged
blood
collection set (Butterfly). - If a vacuum tube butterfly must be used, the first tube in
the
series will be under-filled. Collect a “discard citrate
tube”, and then collect the citrate tube used for testing.
This will ensure the proper blood to anticoagulant ratio. - If using a syringe, use a BD Blood Transfer Device to
transfer
blood from the syringe to the citrate tube within one minute of
collection. Hold the syringe with the plunger in the up-right
position and allow the vacuum present to fill the tube. Before
collection, be sure the syringe plunger is pushed to the top of the
syringe. The transfer device may pop up and needs to be
held down to completely fill tube to between fill
lines. - Do not “force fill” blood into a vacuum tube.
This
may result in activation of the platelets possibly leading to
erroneous results. Allow the vacuum present to fill the tube. - Do not remove vacuum tube cap to add blood.
- Specimens should be obtained from a single venipuncture
with
minimal tissue trauma. Never combine multiple blood collections
into a single vacuum tube. - Never re-use a syringe that was used for a pic line saline
flush.
- It is not recommended to use a winged
blood
Refer to the “Citrated Plasma for Coagulation
Studies” protocol
in the Quality Assurance Manual.
Reference Values
200-393 mg/dL
-
Critical value: < 80 mg/dL
Performance
Testing is performed Monday through Sunday.
Routine orders: Typically completed within 4 hours
Expedite orders: Typically completed within 1 hour after the
specimen arrives at the testing site.
STAT orders: Typically completed within 30 minutes
after
the specimen arrives at the testing site.
Useful For
Detecting increased or decreased fibrinogen (factor I)
concentration of acquired or congenital origin
Monitoring severity and treatment of disseminated
intravascular
coagulation and fibrinolysis
LOINC Code Information
3255-7 Fibrinogen [Mass/Volume] In Platelet Poor Plasma By
Coagulation Assay
CPT Code Information
85384 Coagulopathy Testing: Fibrinogen Activity