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Test Code E2 Estradiol, Serum, LAB523

Methodology

Immunoenzymatic Chemiluminescent Assay

Performing Laboratory

St. Luke's Hospital, Cedar Rapids

Specimen Requirements

Container/Tube:

 

Preferred: Green top (Lithium Heparin)

Acceptable:  Red-top serum, Gold top serum

Specimen: 0.5 mL

Minimum:  0.015 mL

 

Stability: 

  • Room temperature:  24 hours.  Samples on analyzer should be analyzed within 2 hrs
  • Refrigeration (2°-8°C):  48 hours
  • Frozen (≤ -20°C): 6 months.  Freeze samples only once and mix thoroughly after thawing. 

 

Transport Temperature: Refrigerated 

Reference Values

Females >10 years

pg/mL

Female non-pregnant value

 

  Follicular phase (-12 to -4 days)

12 – 233

  Ovulation phase

41-398

  Luteal Phase 

22-341

Post-menopausal females (untreated)

<5-138

 

 

 

 

 

 

 

 

 

Pregnancy 1st Trimester: 154-323 pg/mL

Pregnancy 2nd Trimester 1561-21280 pg/mL

Pregnancy 3rd Trimester  8525- >30000 pg/mL

Children (1-10 years) : Female 6-27 pg/mL  Male <5-20 pg/mL

 

 

Day(s) Test Set Up

Monday-Sunday

Test Classification and CPT Coding

82670

Useful For

Elevated estradiol levels in females may also result from primary or secondary ovarian hyperfunction.  Very high estradiol levels are found during the induction of ovulation1 for assisted reproduction therapy or in pregnancy. Decreased estradiol levels in females may result from either the lack of ovarian synthesis (primary ovarian hypofunction and menopause) or a lesion in the hypothalamus-pituitary axis (secondary ovarian hypofunction). Estradiol levels are normally low in males. Elevated estradiol levels in males may be due to increased aromatization of androgens, resulting in gynecomastia.

Analytic Time

Stat 1 hr within receipt

Routine 2 hrs within receipt