Test Code E2 Estradiol, Plasma or Serum, LAB523
Methodology
Immunoenzymatic Chemiluminescent Assay
Performing Laboratory
St. Luke's Hospital, Cedar Rapids
Specimen Requirements
Container/Tube:
Preferred: Gold-top serum
Acceptable: Red-top serum, Green-top (heparin) tube, Lavender-top (EDTA) plasma
Specimen: 0.5 mL of serum or plasma
Minimum: 0.3 mL of serum or plasma
Stability:
- Room temperature: 20 hours. Do not use samples that have been stored at room temperature for longer than 20 hours.
- Refrigeration (2°-8°C): 48 hours
- Frozen (≤ -20°C): 6 months. Freeze samples only once and mix thoroughly after thawing.
Transport Temperature: Refrigerated preferred, ambient for 20 hours acceptable
Reference Values
Tanner Stage/Age |
Male pg/mL |
Female pg/mL |
I |
≤13 |
≤20 |
II |
≤16 |
≤24 |
III |
≤26 |
≤60 |
IV |
≤38 |
15 – 85 |
V |
10 – 40 |
15 – 350 |
≥18 years |
10 – 40 |
15 – 350 *See Table Below |
Females >12 years |
pg/mL |
Female non-pregnant value |
|
Follicular phase (-12 to -4 days) |
20 – 144 |
Midcycle (-3 to +2 days) |
64 – 357 |
Luteal Phase (+4 to +12 days) |
56 – 214 |
Post-menopausal females (untreated) |
<32 |
Day(s) Test Set Up
Tuesday and Friday
STAT testing available Monday through Friday, day shift only
Test Classification and CPT Coding
82670
Useful For
Elevated estradiol levels in females may also result from primary or secondary ovarian hyperfunction. Very high estradiol levels are found during the induction of ovulation1 for assisted reproduction therapy or in pregnancy. Decreased estradiol levels in females may result from either the lack of ovarian synthesis (primary ovarian hypofunction and menopause) or a lesion in the hypothalamus-pituitary axis (secondary ovarian hypofunction). Estradiol levels are normally low in males. Elevated estradiol levels in males may be due to increased aromatization of androgens, resulting in gynecomastia.
Analytic Time
2-4 days upon receipt