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Test Code CULFBM Fibroblast Culture for Molecular Testing, Chorionic Villi/Products of Conception

Additional Codes



Useful For

Producing cell cultures that can be used for genetic analysis

Method Name

Cell Culture

Reporting Name

Fibroblast Culture for Genetic Test

Specimen Type


Ordering Guidance

This test is most useful for chorionic villus sampling and products of conception specimens.


This test is not appropriate for culturing other tissue specimens such as skin biopsies. If this test is ordered on other tissue types, the test will be canceled and FIBR/ Fibroblast Culture, Tissue will be performed as the appropriate test.

Shipping Instructions

Advise Express Mail or equivalent if not on courier service

Necessary Information

Provide a reason for referral with each specimen. The laboratory will not reject testing if this information is not provided, but appropriate testing and interpretation may be compromised or delayed.


The additional tests desired must be indicated on the request form that accompanies the specimen.

Specimen Required

Submit only 1 of the following specimens:


Specimen Type: Autopsy

Supplies: Hank's Solution (T132)

Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline

Specimen Volume: 4-mm diameter

Collection Instructions:

1. Wash biopsy site with an antiseptic soap.

2. Thoroughly rinse area with sterile water.

3. Do not use alcohol or iodine preparations.

4. Biopsy specimens are best taken by punch biopsy to include full thickness of dermis.


Specimen Type: Chorionic villi

Supplies: CVS Media (RPMI) and Small Dish (T095)

Container/Tube: 15 mL tube containing 15 mL of transport media

Specimen Volume: 20-30 mg

Collection Instructions:

1. Collect specimen by the transabdominal or transcervical method.

2. Transfer the chorionic villi specimen to a Petri dish containing transport medium.

3. Using a stereomicroscope and sterile forceps, assess the quality and quantity of the villi and remove any blood clots and maternal decidua.


Specimen Type: Products of conception or stillbirth

Supplies: Hank's Solution (T132)

Container/Tube: Sterile container with sterile Hank's balanced salt solution, Ringer's solution, or normal saline

Specimen Volume: 1 cm(3) of placenta (including 20 mg of chorionic villi) and a 1 cm(3) biopsy specimen of muscle/fascia from the thigh

Collection Instructions: If a fetus cannot be specifically identified, collect 50 mg villus material or tissue that appears to be of fetal origin.

Additional Information: Do not send entire fetus.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Refrigerated (preferred)

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Day(s) Performed

Monday through Friday

Report Available

41 to 42 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

Not Applicable

CPT Code Information



LOINC Code Information

Test ID Test Order Name Order LOINC Value
CULFB Fibroblast Culture for Genetic Test 96300-9


Result ID Test Result Name Result LOINC Value
52327 Result Summary 50397-9
52329 Interpretation 69965-2
52328 Result 82939-0
CG770 Reason for Referral 42349-1
CG899 Specimen 31208-2
52331 Source 31208-2
52332 Method 85069-3
54625 Additional Information 48767-8
52333 Released By 18771-6


Final Disposition of Fetal/Stillborn Remains (if fetal specimen is sent) in Special Instructions. Only required for products of conception or stillbirth specimen.

Secondary ID


Testing Algorithm

This processing test is for culturing chorionic villus or products of conception specimens for cytogenetic, molecular genetic, or other testing. No analysis or interpretation of results is performed.


Once confluent flasks are established, the cultures are sent to other laboratories, either within Mayo Clinic Laboratories or to external sites, based on the specific testing requested.

Specimen Minimum Volume

See Specimen Required

Reference Values

Not applicable