Test Code SEMBM Semen Analysis with Strict Morphology, Semen
Additional Codes
MAYO: SEMB
EPIC: LAB4501
Useful For
Determining male fertility status
Selecting the most cost-effective therapy for treating male-factor infertility
Quantifying the number of germinal and WBCs per mL of semen
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
FER | Semen Analysis | No | Yes |
MSTC | Strict Criteria Sperm Morphology | No | Yes |
Reporting Name
Semen Analysis with Strict MorphologySpecimen Type
SemenAdvisory Information
Semen analysis specimens submitted to Mayo Clinic Laboratories are not acceptable for fructose testing due to the use of dilution media. See FROS / Fructose, Semen or Seminal Plasma for specimen requirements for fructose testing in azoospermia patients.
Submit separate specimen to rule-out ejaculatory duct blockage. Positive result indicates no blockage.
Shipping Instructions
Specimen must arrive within 24 hours of collection. Send specimen Monday through Thursday only and not the day before a holiday. If holiday falls on a Saturday, holiday will be observed on the preceding Friday. Sunday holidays are observed on the following Monday. Specimen should be collected and packaged as close to shipping time as possible. Laboratory does not perform testing on weekends.
Necessary Information
Include the following information:
-Semen volume (required)
-Viscosity
-pH
-Appearance (color)
-Number of days of sexual abstinence
Specimen Required
Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.
Supplies: Semen Analysis Kit - Dilution Media (T178)
Specimen Volume: Total ejaculate
Collection Instructions:
1. After collection, allow the specimen to liquefy for 1 hour.
2. Measure the volume.
3. Place the specimen into media within 1 hour.
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Semen | Ambient | 36 hours |
Reject Due To
All specimens will be evaluated by Mayo Clinic Laboratories for test suitability.Reference Values
SEMEN ANALYSIS
Appearance: normal
Volume: ≥1.5 mL
pH: ≥7.2
Motile/mL: ≥6.0 x 10(6)
Sperm/mL: ≥15.0 x 10(6)
Motility: ≥40%
Grade: ≥2.5
Note: Multiple laboratory studies have indicated that semen parameters for motility and grade on average retain 80% of original parameters when our shipping method is used for transport. Using these averages, samples with 32% to 39% motility and grade of 2 may be in the normal range if testing was performed shortly after collection. Therefore, these borderline patients may need to collect another sample at a local fertility center to verify fertility status
Motile/ejaculate: ≥9.0 x 10(6)
Viscosity: ≥3.0
Agglutination: ≥3.0
Supravital: ≥58% live
Fructose: positive
Note: Fructose testing cannot be performed on semen analysis specimens shipped through Mayo Clinic Laboratories. If patient is azoospermic, refer to FROS / Fructose, Semen or Seminal Plasma. Submit separate specimen to rule-out ejaculatory duct blockage. Positive result indicates no blockage.
STRICT MORPHOLOGY
Normal forms: ≥4.0% normal oval sperm heads
Germ cells: <4 x 10(6) (normal)
≥4 x 10(6)/mL (elevated germinal cells in semen are of unknown clinical significance)
WBC: <1 x 10(6) (normal)
≥1 x 10(6)/mL (elevated white blood cells in semen are of questionable clinical significance)
Day(s) and Time(s) Performed
Monday through Friday; 3 p.m.
Send specimen Monday through Thursday only and not the day before a holiday.
Performing Laboratory

CPT Code Information
89310-Semen Analysis
89398-Strict Criteria Sperm Morphology
If both components performed,
89322-Semen Analysis with Strict Morphology
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
SEMB | Semen Analysis with Strict Morphology | In Process |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
OVAL2 | Strict Morph NL | 10622-9 |
ACRSM | Acrosom Defect | 66494-6 |
ABSTN | Abstinence | 10587-4 |
CLST1 | Collection Site | 56816-2 |
HDSAB | Head Shape Abnormal | 66495-3 |
HDZAB | Head Size Abnormal | 66496-1 |
TY | Study Type | 54453-6 |
CNTN | Container Type | 74384-9 |
MD | Midpiece Defect | 10603-9 |
TAILD | Tail Defect | 10604-7 |
DBLF | Double Forms | 66497-9 |
MULTI | Multiple Defects | 66498-7 |
APP3 | Appearance | 13359-5 |
VL53 | Semen Volume | 3160-9 |
GERM3 | Germ Cells/mL | 10576-7 |
WBC6 | WBC/mL | 10579-1 |
PH1 | pH | 2752-4 |
CMT56 | Comment | 48767-8 |
MOTML | Motile/mL | 42531-4 |
SPML | Sperm/mL | 9780-8 |
MOTY | Motility | 6800-7 |
GR2 | Grade | 13942-8 |
MOTEJ | Motile/Ejaculate | 6800-7 |
VISC | Viscosity | 32789-0 |
AGGLU | Agglutination | 33217-1 |
STAIN | Supravital Stain | 4466-9 |
FRCT | Fructose | 13943-6 |
CMT45 | Comment | 48767-8 |
Specimen Minimum Volume
A minimum count is needed. Lab will determine.
Method Name
FER: Manual
MSCT: Kruger Criteria Strict Morphology