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Test Code PTHOR Parathyroid Hormone (PTH), Intraoperative LAB3245

Important Note

Human Anti-Mouse Antibodies (HAMA) may be present in samples from patients who have received immunotherapy utilizing monoclonal antibodies. Additionally, other heterophile antibodies such as human anti-goat antibodies may be present in patient samples. Manufacturers formulate assays to minimize the effects of these antibodies; however careful evaluation of patient results must be done when patients are known to have such antibodies.

Test Method

Two-Site Immunoenzymatic (“Sandwich”) Assay; Beckman


Testing is performed Monday through Sunday.

Typically completed within 30 minutes after the
specimen arrives at the testing site.

Useful For

Interoperative mode testing in patients under-going
parathyroidectomy for primary hyperparathyroidism,

The following practices are recommended:

  • Baseline samples should be drawn at pre-operation/exploration
    and pre-excision.
  • Ordering pattern for the PTH-Intraoperative is typically
    baseline and then samples starting at about 10 minutes following
    resection and repeated until a 50% or better reduction from the
    baseline value is

Specimen Requirement

0.5 mL heparin plasma is required.

CPT Code Information

83970: Chemistry: Parathormone (parathyroid hormone)

Reference Values

15-65 pg/mL

50% or better reduction from baseline value.

LOINC Code Information

72203-3 Parathyrin.Intact Intraoperative Panel-Serum Or

Additional Specimen Collection Information

Collect blood in a lithium heparin, green-top. PST are not
acceptable. Plasma should be separated from contact with the
cells within 2 hours of collection. Specimens not centrifuged
within 4 hours of collection may be rejected. Refrigerate the
specimen if unable to assay within 8 hours of collection. Samples
with ≥3+ lipemia must be cleared prior to analysis. All
samples collected before and during the procedure must be collected
from the same site peripheral to surgery. Typically this will be
from an arterial line.