Sign in →

Test Code PPFEM Protoporphyrins, Fractionation, Whole Blood

Additional Codes



Reporting Name

Protoporphyrins, Fractionation, WB

Useful For

Evaluating patients with possible diagnoses of erythropoietic protoporphyria or X-linked dominant protoporphyria


Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria

Testing Algorithm

This test should not be ordered in conjunction with the PEE / Porphyrins Evaluation, Whole Blood.


The following algorithms are available in Special Instructions:

-Porphyria (Acute) Testing Algorithm

-Porphyria (Cutaneous) Testing Algorithm

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole blood

Ordering Guidance

This test is for assessment for protoporphyria. The preferred test for lead toxicity in children is blood lead. For more information see PBDV / Lead, Venous with Demographics, Blood or PBDC / Lead, Capillary, with Demographics, Blood. The preferred screening test for suspicion of a hepatic porphyria is urine porphyrins. For more information see PQNRU / Porphyrins, Quantitative, Random, Urine.

Necessary Information

Include a list of medications the patient is currently taking.

Specimen Required

All porphyrin tests on whole blood can be performed on 1 tube.


Patient Preparation: Patient should abstain from alcohol for 24 hours.


Preferred: Green top (sodium heparin)

Acceptable: Dark blue top (metal free heparin) or green top (lithium heparin)

Specimen Volume: Full tube

Collection Instructions: Immediately place specimen on wet ice.

Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Refrigerated 7 days

Reference Values


<20 mcg/dL



<60 mcg/dL

Day(s) Performed

Monday, Wednesday, Friday

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
PPFE Protoporphyrins, Fractionation, WB 94490-0


Result ID Test Result Name Result LOINC Value
2327 Zinc-Complexed Protoporphyrin 2895-1
2326 Free Protoporphyrin 94491-8
29511 Interpretation 59462-2

Report Available

2 to 3 days

Reject Due To

Gross hemolysis Reject

Method Name

High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection