Test Code NICOUM Nicotine and Metabolites, Random, Urine
Additional Codes
MAYO: NICOU
EPIC: LAB3971
Reporting Name
Nicotine and Metabolites, UUseful For
Monitoring tobacco use
Monitoring patients on nicotine-replacement therapy for concurrent use of tobacco products
Performing Laboratory

Specimen Type
UrineSpecimen Required
Supplies: Aliquot Tube, 5 mL (T465)
Container/Tube: Plastic, 5 mL, aliquot tube
Specimen Volume: 3 mL
Collection Instructions:
1. Collect a random urine specimen.
2. No preservative.
Specimen Minimum Volume
2 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Urine | Ambient (preferred) | 28 days | |
Frozen | 365 days | ||
Refrigerated | 28 days |
Reference Values
Non-tobacco user with no passive exposure:
NICOTINE
<5.0 ng/mL
COTININE
<5.0 ng/mL
ANABASINE
<2.0 ng/mL
NORNICOTINE
<2.0 ng/mL
Day(s) and Time(s) Performed
Monday through Sunday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
80323
G0480 (if appropriate)
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
NICOU | Nicotine and Metabolites, U | 43125-4 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
82510 | Nicotine | 3854-7 |
21321 | Cotinine | 10366-3 |
21324 | Nornicotine | 33917-6 |
21323 | Anabasine | 33915-0 |
Reject Due To
Gross hemolysis | OK |
Gross icterus | OK |
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.