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Test Code DATG Direct Antiglobulin Test (LAB274)

Test Method

AABB Standard: Agglutination of red cells with an antiserum
represents the presence of the corresponding antigen in the red
cells; (AABB Technical manual. 14th edition.
Edited by V Vengelen-Tyler. Bethesda, MD, American Association of
Blood Banks 2002)

Performance

Testing is performed Monday through Sunday.

Routine: Typically completed within 4 hours after the
specimen arrives at the testing site.

STAT: Typically completed within 1 hour after the specimen
arrives at the testing site.

Specimen Requirement

The only acceptable specimens are: Red blood cells collected in
EDTA or Cord Blood collected in EDTA, clot tubes, or tubes with no
additive.  A capillary or micro sample may be collected in an
EDTA microtainer. Serum/Plasma gel tubes are NOT acceptable.

Useful For

Detecting possible ABO or Rh(D) incompatibility with mother’s
blood.

Demonstrates in vivo coating of RBCs with IgG.

Reference Values

Direct Antiglobulin Test:

  • IgG: Negative
  • If positive results are called to the provider

CPT Information

86880 Direct Antiglobulin Test