Test Code COR60M Cortisol Stimulation 60 minutes
Methodology
Chemiluminescence
Performing Laboratory
St. Luke's Hospital Cedar Rapids
Specimen Requirements
Cortrosyn is available from the pharmacy or Omnicell.
Baseline - REQUIRED (60-minute post dose specimen must also be submitted.)
Baseline
Submit specimen according to guidelines in procedure "Cortisol Stimulation Baseline, LAB2385)
60-Minute Post Dose
Submit only 1 of the following specimens:
Container/Tube:
Preferred: Green-top (lithium heparin) tube
Acceptable: Gold-top serum gel tube or Plain Red-top serum tube, lavender EDTA plasma
Specimen Volume: 170 uL of plasma or serum
Stability: Due to possible evaporation effects, samples and calibrators on the analyzers should be analyzed/measured within 2 hours. Stable for 24 hours at 20‑25 °C, 4 days at 2‑8 °C, 12 months at ‑20 °C (± 5 °C). Freeze only once.
Collection Instructions: Draw post dose specimen 60 minutes after a 0.25 mg dose of Cortrosyn is injected IM.
Note: Label specimen appropriately (60 minutes post Cortrosyn injection).
Reference Values
CORTISOL
Baseline (AM): 4.8-19.5 mcg/dL
Cortisol 60 min: Should show an approximate doubling of baseline cortisol level
Day(s) Test Set Up
Monday through Sunday
Test Classification and CPT Coding
80400
Useful For
The ACTH stimulation test is useful in the screening of patients presumed to have adrenocortical insufficiency.
Report Available
1 hour