Sign in →

Test Code BASFM Bile Acids, Fractionated and Total, Serum

Additional Codes



Advisory Information

This bile acid test is useful in diagnosing intrahepatic cholestasis of pregnancy.


Do not use this assay for the diagnosis of peroxisomal biogenesis disorders (see BAIPD / Bile Acids for Peroxisomal Disorders, Serum) or inborn errors of bile acid metabolism.

Specimen Required

Patient Preparation: Patient must be fasting for 12 to 14 hours.

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL


If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.

Useful For

Measuring tauro- and glycol-conjugated and unconjugated bile acid constituents in serum


Monitoring patients receiving bile acid therapy, such as cholic acid, deoxycholic acid, or ursodeoxycholic acid


Aiding in the evaluation of liver function; evaluation of liver function changes before the formation of more advanced clinical signs of illness such as icterus


Determining hepatic dysfunction as a result of chemical and environmental injury


Indicating hepatic histological improvement in chronic hepatitis C patients responding to interferon treatment


Indicating intrahepatic cholestasis of pregnancy

Testing Algorithm

See Ordering Guide: Bile Acid-Associated Tests in Special Instructions.

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Bile Acids, Fractionated and Tot, S

Specimen Type


Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 90 days
  Ambient  90 days
  Frozen  90 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Reference Values


Normal (nmol/mL)

Total cholic acid


Total chenodeoxycholic acid


Total deoxycholic acid


Total ursodeoxycholic acid


Total bile acids









Day(s) and Time(s) Performed

Monday, Wednesday, Thursday, Friday; 8 a.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
BAFS Bile Acids, Fractionated and Tot, S 43130-4


Result ID Test Result Name Result LOINC Value
35796 Total Cholic acid 30518-5
35797 Total Chenodeoxycholic acid 30519-3
35798 Total Deoxycholic acid 30520-1
35799 Total Ursodeoxycholic acid 55159-8
35800 Total bile acids 14628-2