Test Code TPNUVM Thiopurine Methyltransferase (TPMT) and Nudix Hydrolase (NUDT15) Genotyping, Varies
Additional Codes
MAYO: TPNUV
EPIC: LAB5338
Advisory Information
For thiopurine methyltransferase (TPMT) enzyme activity testing, order TPMT3 / Thiopurine Methyltransferase (TPMT) Activity Profile, Erythrocytes.
Specimen Required
Multiple genotype tests can be performed on a single specimen after a single extraction. See Multiple Genotype Test List in Special Instructions for a list of tests that can be ordered together.
Submit only 1 of the following specimens:
Specimen Type: Whole blood
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send specimen in original tube.
Specimen Stability Information: Ambient (preferred) 9 days/Refrigerated 30 days
Specimen Type: Saliva
Patient Preparation: Patient should not eat, drink smoke, or chew gum 30 minutes prior to collection.
Supplies: Saliva Swab Collection Kit (T786)
Collection Instructions: Collect and send specimen per kit instructions.
Specimen Stability Information: Ambient 30 days
Specimen Type: DNA
Container/Tube: 2 mL screw top tube
Specimen Volume: 100 mcL
Collection Instructions:
1. The preferred volume is 100 mcL at a concentration of 50 ng/mcL.
2. Include concentration and volume on tube.
Specimen Stability Information: Frozen (preferred)/Ambient/Refrigerated
Forms
1. New York Clients-Informed consent is required. Document on the request form or electronic order that a copy is on file. The following documents are available in Special Instructions:
-Informed Consent for Genetic Testing (T576)
-Informed Consent for Genetic Testing-Spanish (T826)
2. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Neurology Specialty Testing Client Test Request (T732)
-Gastroenterology and Hepatology Client Test Request (T728)
-Therapeutics Test Request (T831)
Secondary ID
65160Useful For
Predicting potential for toxicity to thiopurine drugs (6-mercaptopurine, 6-thioguanine, and azathioprine)
Special Instructions
Method Name
Real-Time Polymerase Chain Reaction (PCR) With Allelic Discrimination Analysis
Reporting Name
TPMT and NUDT15 GenotypeSpecimen Type
VariesSpecimen Minimum Volume
Blood: 0.4 mL
Saliva: 1 swab
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Varies | Varies |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. |
Reference Values
An interpretive report will be provided.
Day(s) and Time(s) Performed
Monday through Friday; 8 a.m.
Performing Laboratory

Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
0034U
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
TPNUV | TPMT and NUDT15 Genotype | 93193-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
BA0211 | TPMT Genotype | 41048-0 |
BA0212 | TPMT Phenotype | 79713-4 |
BA0213 | NUDT15 Genotype | 93194-9 |
BA0214 | NUDT15 Phenotype | 93195-6 |
BA0215 | Interpretation | 69047-9 |
BA0216 | Additional Information | 48767-8 |
BA0217 | Method | 49549-9 |
BA0218 | Disclaimer | 62364-5 |
BA0219 | Reviewed by | 18771-6 |