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Test Code ADM13 ADAMTS13 Activity and Inhibitor Profile, Plasma

Reporting Name

ADAMTS13 Activity and Inhibitor Profile

Useful For

Assisting with the diagnosis of congenital or acquired thrombotic thrombocytopenic purpura

Profile Information

Test ID Reporting Name Available Separately Always Performed
ADMFX ADAMTS13 Activity Assay No Yes
ADMIN ADAMTS13 Interpretation No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ADMIS ADAMTS13 Inhibitor Screen No No
ADMBU ADAMTS13 Inhibitor Bethesda Titer No No

Testing Algorithm

Testing begins with the ADAMTS-13 activity assay to evaluate the percent activity. If the ADAMTS-13 activity is less than 30%, an inhibitor screen will be performed to look for specific ADAMTS-13 inhibition. If specific inhibition is apparent, the titer of the inhibitor will be determined.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Plasma Na Cit


Shipping Instructions


Send both vials in the same shipping container.



Specimen Required


Patient Preparation: Fasting preferred

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vials

Specimen Volume: 2 mL in 2 plastic vials each containing 1 mL

Collection Instructions:

1. Specimen must be collected prior to replacement therapy.

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing

3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

4. Aliquot plasma (1 mL per aliquot) into 2 separate plastic vials, leaving 0.25 mL in the bottom of centrifuged vial.

5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, below -40° C.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. If priority specimen, mark request form, give reason, and request a call-back.

3. Each coagulation assay requested should have its own vial.


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Reference Values

ADAMTS13 ACTIVITY ASSAY

≥70%

 

ADAMTS13 INHIBITOR SCREEN

Negative

 

ADAMTS13 BETHESDA TITER

<0.4 BU

Day(s) Performed

Monday through Friday, Sunday

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85397

85335 (if appropriate)

85335  (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ADM13 ADAMTS13 Activity and Inhibitor Profile 53622-7

 

Result ID Test Result Name Result LOINC Value
61211 ADAMTS13 Activity Assay 53622-7
34586 ADAMTS13 Interpretation 69049-5

Report Available

1 to 3 days

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Method Name

Fluorescence Resonance Energy Transfer (FRET)

Forms

1. Coagulation Patient Information (T675)

2. If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Coagulation Test Request (T753)

-Renal Diagnostics Test Request (T830)

Secondary ID

61212